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Citation
Tags
HERO ID
1454265
Reference Type
Journal Article
Title
A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone
Author(s)
Martin, CH; Dhamankar, H; Tseng, HC; Sheppard, MJ; Reisch, CR; Prather, KL
Year
2013
Is Peer Reviewed?
1
Journal
Nature Communications
EISSN:
2041-1723
Volume
4
Page Numbers
1414
Language
English
PMID
23361005
DOI
10.1038/ncomms2418
Abstract
The replacement of petroleum feedstocks with biomass to produce platform chemicals requires the development of appropriate conversion technologies. 3-Hydroxy-γ-butyrolactone has been identified as one such chemical; however, there are no naturally occurring biosynthetic pathways for this molecule or its hydrolyzed form, 3,4-dihydroxybutyric acid. Here we design a novel pathway to produce various chiral 3-hydroxyacids, including 3,4-dihydroxybutyric acid, consisting of enzymes that condense two acyl-CoAs, stereospecifically reduce the resulting β-ketone and hydrolyze the CoA thioester to release the free acid. Acetyl-CoA serves as one substrate for the condensation reaction, whereas the second is produced intracellularly by a pathway enzyme that converts exogenously supplied organic acids. Feeding of butyrate, isobutyrate and glycolate results in the production of 3-hydroxyhexanoate, 3-hydroxy-4-methylvalerate and 3,4-dihydroxybutyric acid+3-hydroxy-γ-butyrolactone, respectively, molecules with potential uses in applications from materials to medicines. We also unexpectedly observe the condensation reaction resulting in the production of the 2,3-dihydroxybutyric acid isomer, a potential value-added monomer.
Tags
IRIS
•
n-Butanol
Database searches
Pubmed
Source – January 2013 (private)
Pubmed - 1/2013
Merged reference set - 1/2013
Excluded (not pertinent)
Not chemical specific
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