Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1454876
Reference Type
Journal Article
Title
Selective production of acetone during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MAceT113
Author(s)
Berzin, V; Kiriukhin, M; Tyurin, M
Year
2012
Is Peer Reviewed?
1
Journal
Letters in Applied Microbiology
ISSN:
0266-8254
EISSN:
1472-765X
Volume
55
Issue
2
Page Numbers
149-154
Language
English
PMID
22642684
DOI
10.1111/j.1472-765X.2012.03272.x
Web of Science Id
WOS:000306735200009
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864040646&doi=10.1111%2fj.1472-765X.2012.03272.x&partnerID=40&md5=5aa0112df6cb2c10a2cf7a0cafec2e68
Exit
Abstract
AIMS:
To engineer acetogen biocatalyst capable of fermenting synthesis gas blend to acetone as the only liquid carbonaceous product.
METHODS AND RESULTS:
The metabolic engineering comprised inactivation of phosphotransacetylase via integration of a cassette comprising synthetic genes erm(B), thiolase and HMG-CoA synthase. Acetaldehyde dehydrogenase was inactivated via integration of a cassette consisting of synthetic genes cat, HMG-CoA lyase and acetoacetate decarboxylase. The engineered biocatalyst Clostridum sp. MAceT113 lost production of 253 mmol l(-1) ethanol and 296 mmol l(-1) acetate and started producing 1.8 mol l(-1) acetone in single-stage continuous syngas fermentation.
CONCLUSIONS:
The acetone concentration in culture broth is economical for bulk manufacture because it is about twenty times of that achieved with known acetone-butanol-ethanol fermentation of sugars.
SIGNIFICANCE AND IMPACT OF THE STUDY:
The process shows the opportunity to produce acetone from synthesis gas at concentrations comparable with production of acetone from products of petroleum cracking. This is the first report on elimination of acetate and acetaldehyde production and directing carbon flux from Acetyl-CoA to acetone via a non-naturally occurring in acetogen acetone biosynthesis pathway identified in eukaryotic organisms.
Keywords
article; Acetone; Biofuels; Bioreactors; Clostridium; Fermentation; Hydroxymethylglutaryl-CoA Synthase; Industrial Microbiology; Metabolic Engineering; acetaldehyde dehydrogenase; acetates; biosynthesis; cracking; ethanol; manufacturing; petroleum; synthetic genes
Tags
•
n-Butanol
Database searches
Pubmed
Source – January 2013 (private)
Pubmed - 1/2013
Merged reference set - 1/2013
Excluded (not pertinent)
Not chemical specific
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity