US EPA

1456867 

Journal Article 

Composition of anthocyanins in mulberry and their antioxidant activity 

Du, Q; Zheng, J; Xu, Y 

2008 

Yes 

Journal of Food Composition and Analysis
ISSN: 0889-1575
EISSN: 1096-0481 

21 

5 

390-395 

English 

10.1016/j.jfca.2008.02.007 

WOS:000257484700005 

Anthocyanins in the fruits of mulberry (Morus alba L.) were extracted and separated by highspeed counter-current chromatography (HSCCC) using a biphasic solvent system composed of methyl tert-butyl ether-n-butanol-acetonitrile-water-trifluoroacetic acid (1:3:1:5:0.001) to yield five anthocyanins: cyanidin 3-O-(6"-O-alpha-rhamnopyranosyl-beta-D-glucopyranoside) (C3RG), cyaniclin 3-0-(6"-O-arhamnopyranOSyl-beta-D-galactopyranoside) (C3RGa), cyaniclin 3-O-beta-D-glucopyranoside (C3G), cyaniclin 3-O-beta-D-galactopyranoside (C3Ga) and cyanidin 7-O-beta-D-glucopyranoside (C7G), respectively. The five compounds were identified by ESI-MS and one/two-dimensional NMR spectra. The antioxidant activity of crude mulberry anthocyanins (CMA), C3G, C3Ga, C7C, C3RG and C3RGa was investigated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method.-The results showed that CMA, C3G, C3Ga, C3G and C7G have higher scavenging ability on DPPH. At the concentration of 0.10 mg/mL, the DPPH radical scavenging rates of C3G, C3Ga and C7G were about 88% of vitamin C, while C3RG and C3RGa were about 60% of it. CMA had the same DPPH radical scavenging rate as vitamin C or the five anthocyanin monomers when the concentration reached 0.40 mg/mL, which shows that CMA is an excellent antioxidant agent. (C) 2008 Elsevier Inc. All rights reserved. 

mulberry; Morus alba L.; anthocyanins; composition; antioxidant activity; NMR; spectroscopy; food composition; food analysis