US EPA

1458518 

Journal Article 

Estrogenic Effects of Various Extracts from Chamdanggui (Angelica gigas Nakai) and Sogdan (Phlomis umbrosa Turcz) 

Bae, InY; Lee, JiY; Kwak, BoY; Lee, HGyu 

2011 

1 

Food Science and Biotechnology
ISSN: 1226-7708
EISSN: 2092-6456 

20 

4 

1113-1118 

10.1007/s10068-011-0151-1 

WOS:000294546400031 

http://www.springerlink.com/index/10.1007/s10068-011-0151-1
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The various extracts from chamdanggui (Angelica gigas Nakai)
and sogdan (Phlomis umbrosa Turcz) were evaluated for estrogenic activity and characterized
according to HPLC profile. Chamdanggui and sogdan were individually extracted with 4 solvents
(hot water, 70% ethanol, n-butanol, and dichloromethane) of differing polarities. Estrogenic
activity was determined by E-screen using an estrogen-dependent MCF-7 BUS cell. Although almost
all extracts showed estrogenic effects in a concentration-dependent manner, the hot water extract
from chamdanggui (250 mu g/mL) had the higher effect (138%). Among 90 fractions using HPLC
separation of the hot water extract from chamdanggui, fraction 21 and 28 produced the highest
estrogenic effects of 178 and 163% at 10 mu g/mL, respectively. The results imply that the hot
water extract from chamdanggui could be useful as an alternative hormone replacement therapy. 

Angelica gigas Nakai; Phlomis umbrosa Turcz; estrogenic effect; MCF-7 BUS cell