US EPA

1463449 

Journal Article 

Rapid determination of water- and fat-soluble vitamins with microemulsion electrokinetic chromatography 

Yin, C; Cao, Y; Ding, S; Wang, Yun 

2008 

Yes 

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 

1193 

1-2 

172-177 

English 

18440539 

10.1016/j.chroma.2008.04.016 

WOS:000256558600024 

A rapid, reliable and reproducible method based on microemulsion electrokinetic chromatography (MEEKC) for simultaneous determination of 13 kinds of water- and fat-soluble vitamins has been developed in this work. A novel microemulsion system consisting of 1.2% (w/w) sodium lauryl sulphate (SDS), 21% (v/v) 1-butanol, 18% (v/v) acetonitrile, 0.8% (w/w) n-hexane, 20mM borax buffer (pH 8.7) was applied to improve selectivity and efficiency, as well as shorten analysis time. The composition of microemulsion used as the MEEKC running buffer was investigated thoroughly to obtain stable separation medium, as well as the optimum determination conditions. Acetonitrile as the organic solvent modifier, pH of the running buffer and 1-butanol as the co-surfactant played the most important roles for the separation of the fat-soluble vitamins, water-soluble vitamins and stabilization of system, respectively. The 13 water- and fat-soluble vitamins were baseline separated within 30 min. The system was applied to determine water- and fat-soluble vitamins in commercial multivitamin pharmaceutical formulation, good accuracy and precision were obtained with recoveries between 97% and 105%, relative standard derivations (RSDs) less than 1.8% except vitamin C, and acceptable quantitative results corresponding to label claim. 

microemulsion electrokinetic; chromatography; vitamin; microemulsion