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HERO ID
1463840
Reference Type
Journal Article
Title
Simultaneous microemulsion liquid chromatographic analysis of fat-soluble vitamins in pharmaceutical formulations: Optimization using genetic algorithm
Author(s)
Momenbeik, F; Roosta, M; Nikoukar, AliA
Year
2010
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography A
ISSN:
0021-9673
EISSN:
1873-3778
Volume
1217
Issue
24
Page Numbers
3770-3773
Language
English
PMID
20444455
DOI
10.1016/j.chroma.2010.04.012
Web of Science Id
WOS:000278563900008
Abstract
An environmentally benign and simple method has been proposed for separation and determination of fat-soluble vitamins using isocratic microemulsion liquid chromatography. Optimization of parameters affecting the separation selectivity and efficiency including surfactant concentration, percent of cosurfactant (1-butanol), and percent of organic oily solvent (diethyl ether), temperature and pH were performed simultaneously using genetic algorithm method. A new software package, MLR-GA, was developed for this purpose. The results indicated that 73.6mM sodium dodecyl sulfate, 13.64% (v/v) 1-butanol, 0.48% (v/v) diethyl ether, column temperature of 32.5 degrees C and 0.02M phosphate buffer of pH 6.99 are the best conditions for separation of fat-soluble vitamins. At the optimized conditions, the calibration plots for the vitamins were obtained and detection limits (1.06-3.69microgmL(-1)), accuracy (recoveries>94.3), precision (RSD<3.96) and linearity (0.01-10mgmL(-1)) were estimated. Finally, the amount of vitamins in multivitamin syrup and a sample of fish oil capsule were determined. The results showed a good agreement with those reported by manufactures.
Keywords
Fat-soluble vitamins; Microemulsion; High-performance liquid chromatography; Genetic algorithm; Pharmaceutical formulations
Tags
IRIS
•
n-Butanol
Database searches
WOS
Source – January 2013 (private)
WOS - 1/2013
Merged reference set - 1/2013
Excluded (not pertinent)
Not chemical specific
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