US EPA

1479818 

Journal Article 

Acid and Alkaline Phosphatase of Serum and Hepatic Tissue of Rats Fed Carcinogens 

Mulay, AS; Firminger, HI 

1952 

Yes 

Journal of the National Cancer Institute
ISSN: 0027-8874
EISSN: 1460-2105 

NIOSH/00021000 

12 

5 

917-927 

Acid and alkaline phosphatase values of serum and hepatic tissue (including hepatoma) of experimental rats fed carcinogens (p-dimethylaminoazobenzene (60117) or 4-dimethylaminobenzene-1-azo-1-naphthalene) were determined chemically in order to document changes in such values preceding or following tumor development and whether such changes may be correlated with histologic changes in the liver during development of a neoplasm. A rise in serum alkaline phosphatase (9001789) was demonstrated in 28 percent of animals bearing tumors of the liver, and there was no appreciable difference between acid phosphatase (9001778) content of blood serum of control, nontumorous, and tumor bearing rats. Acid phosphatase values for hepatic tumor tissue had much wider range and lower mean value than those of normal liver tissue of control rats, 50 percent of values for hepatic tumors being below the range of values for normal liver. Lowest alkaline phosphatase values of hepatic tumors were higher than the highest value of normal liver and highest values were 24 times the mean value of normal liver. Mean value of hepatic tumors was 10 times greater than that of normal livers. Both acid and alkaline phosphatase values for liver-cell type tumor tissue were higher than those for cholangiohepatoma type tissue. The mean alkaline phosphatase value for liver of carcinogen fed rats was twice that of the liver of the control animals. Alkaline phosphatase values above the range of normal liver values were noted in 65 percent of the nontumorous liver of carcinogen fed rats and 75 percent of the tumor free tissue of livers bearing tumors. 

DCN-155822; Carcinogenesis; Histology; Pathology; Azobenzenes; Naphthalenes