US EPA

1482875 

Journal Article 

Biological assay of endrin in leafy vegetables and its confirmation by paper chromatography 

Klein, AK; Laug, EP; Tighe, JF; Ramsey, LL; Mitchell, LC; Kunze, FM 

1956 

Yes 

Journal of the Association of Official Agricultural Chemists
ISSN: 0095-9111 

39 

1 

242-253 

English 

CABI:19560305199 

An experimental procedure is reported by means of which micro quantities of endrin (72208) residues in leafy vegetables were measured. A bioassay, using houseflies as the test medium, determined the amount of insecticide present. Paper chromatography then proved that the insecticidal action was due solely to endrin. Endrin in leafy vegetables was evaluated at the 0.1 and 0.5 ppm levels with an accuracy of approximately 70 and 85 percent. Reproducibility of the fly assay averaged plus/minus 0.027 ppm in extracts containing 0.13 to 1.0 ppm endrin. When 0.10 ppm or less was present, the average deviation was plus/minus 0.014 ppm. The impurity in technical endrin seemed relatively nontoxic to flies and had no observable acute toxicity in rats. Endrin is a central nervous system toxicant with a delayed action. Its LD50 is approximately 25 mg/kg in young male rats. 

69th Annual Meeting of the Association of Official Agricultural Chemists 

October 10-12, 1955