US EPA

2152979 

Journal Article 

Abstract 

Persistence of PCB homologs and isomers in domestic hen and rat 

Baker, FD; Bush, B; TUMASONI.C 

1974 

1 

Toxicology and Applied Pharmacology
ISSN: 0041-008X
EISSN: 1096-0333 

29 

1 

104-105 

English 

WOS:A1974T653800081 

is part of a larger document 3378179 Abstracts of papers for the Thirteenth Annual Meeting of the Society of Toxicology, Washington, D.C. March 10–14, 1974

This study was undertaken to (1) separate, identify, and quantitate the 18 major component of Aroclor 1254 in adult hens and pregnant rats, in the embryos and fetuses, and in the progeny of both species during and after exposure; (2) compare the metabolic patterns of Aroclor 1254 for both species; (3) correlate toxicity and abnormalities with specific isomers; and (4) examine the possible relationship between induced mixed-function oxidase activity and hydroxylation of isomers. Aroclor 1254, emulsified in drinking water, was administered at a level of 6 mg/kg body weight (50 ppm) to hens and to rats over periods of 6 and 9 weeks respectively. After the intake was terminated, the populations were observed for 20 and 13 weeks respectively as the level of PCB declined. Egg yolks, tissues of viable 18-day-old embryos, and surviving 5-day-old chicks were analyzed by thin-layer chromatography and by gas chromatography during the course of the experiment. Data suggest that 4-4’chlorination is more important than degree of chlorination in determining persistence of chlorinated biphenyl in the hen, chick embryo and hatched chick. Results with both hens and rats indicate that fertility was not affected at the level of PCB employed and that the degree, and termination, of embryonic and/or fetal development was related directly to length of exposure. Mixed-function oxidase activity (MFO) on liver microsomal preparations from rat mothers, fetuses and pups was measured by determination of liver microsomal cytochrome P-450 and aniline hydroxylase levels. Preliminary results indicate sex-related MFO response. 

Thirteenth Annual Meeting of the Society of Toxicology 

Washington, D.C. 

March 10–14, 1974