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HERO ID
2236615
Reference Type
Journal Article
Title
Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages
Author(s)
Jung, HA; Jin, SE; Ahn, BR; Lee, CM; Choi, JS
Year
2013
Is Peer Reviewed?
Yes
Journal
Food and Chemical Toxicology
ISSN:
0278-6915
EISSN:
1873-6351
Volume
59
Page Numbers
199-206
Language
English
PMID
23774261
DOI
10.1016/j.fct.2013.05.061
Web of Science Id
WOS:000324280000026
Abstract
Although individual phlorotannins contained in the edible brown algae have been reported to possess strong anti-inflammatory activity, the responsible components of Eisenia bicyclis have yet to be fully studied. Thus, we evaluated their anti-inflammatory activity via inhibition against production of lipopolysaccharide (LPS)-induced nitric oxide (NO) and tert-butylhydroperoxide (t-BHP)-induced reactive oxygen species (ROS), along with suppression against expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), in RAW 264.7 cells. The anti-inflammatory activity potential of the methanolic extract and its fractions of E. bicyclis was in the order of dichloromethane>methanol>ethyl acetate>n-butanol. The strong anti-inflammatory dichloromethane fraction was further purified to yield fucosterol. From the ethyl acetate fraction, six known phlorotannins were isolated: phloroglucinol, eckol, dieckol, 7-phloroeckol, phlorofucofuroeckol A and dioxinodehydroeckol. We found that these compounds, at non-toxic concentrations, dose-dependently inhibited LPS-induced NO production. Fucosterol also inhibited t-BHP-induced ROS generation and suppressed the expression of iNOS and COX-2. These results indicate that E. bicyclis and its constituents exhibited anti-inflammatory activity which might attribute to inhibition of NO and ROS generation and suppression of the NF-κB pathway and can therefore be considered as a useful therapeutic and preventive approach to various inflammatory and oxidative stress-related diseases.
Keywords
Eisenia bicyclis; Fucosterol; Phlorotannin; Anti-inflammation; ROS
Tags
IRIS
•
n-Butanol
Database searches
Pubmed
WOS
•
tert-Butanol
Considered Studies
Electronic Search
•
n-Butanol
Database Searches - March 2014 (private)
Pubmed - 3/2014
WOS – 3/2014
Excluded (not pertinent)
Use in sample prep or assay
•
tert-Butanol
Excluded/ Not on Topic
Other Chemical/Non-tert-butanol
OPPT REs
•
OPPT_1,4-Dioxane_F. Human Health
Total – title/abstract screening
Off topic
•
OPPT_Methylene Chloride_C. Engineering
Total – title/abstract screening
Off topic
•
OPPT_Methylene Chloride_D. Exposure
Total – title/abstract screening
Off topic
•
OPPT_Methylene Chloride_E. Fate
Total – title/abstract screening
Off topic
•
OPPT_Methylene Chloride_F. Human Health
Total – title/abstract screening
Off topic
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