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HERO ID
2279527
Reference Type
Journal Article
Title
Alteration of cholinesterase activity as possible mechanism of silver nanoparticle toxicity
Author(s)
Sinko, G; Vrcek, IV; Goessler, W; Leitinger, G; Dijanosic, A; Miljanic, S
Year
2014
Is Peer Reviewed?
Yes
Journal
Environmental Science and Pollution Research
ISSN:
0944-1344
EISSN:
1614-7499
Volume
21
Issue
2
Page Numbers
1391-1400
Language
English
PMID
23904256
DOI
10.1007/s11356-013-2016-z
Web of Science Id
WOS:000329243700057
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891624633&doi=10.1007%2fs11356-013-2016-z&partnerID=40&md5=f7139cd117609048c4dcfc93f47c29de
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Abstract
Due to their broad-spectrum antimicrobial activity, silver nanoparticles (AgNPs) have been used in a large number of commercial and medical products. Such proliferated AgNP production poses toxicological and environmental issues which need to be addressed. The present study aimed to investigate the effects of AgNPs on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), important enzymes in areas of neurobiology, toxicology and pharmacology. Three different AgNPs, prepared by the chemical reduction using trisodium citrate, hydroxylamine hydrochloride (Cl-AgNPs), and borohydride following stabilization with poly(vinyl alcohol), were purified and characterised with respect to their sizes, shapes and optical properties. Their inhibition potential on AChE and BChE was evaluated in vitro using an enzyme assay with o-nitrophenyl acetate or o-nitrophenyl butyrate as substrates, respectively. All three studied AgNPs were reversible inhibitors of ChEs. Among tested nanoparticles, Cl-AgNP was found to be the most potent inhibitor of both AChE and BChE. Although the detailed mechanism by which the AgNPs inhibit esterase activities remains unknown, structural perturbation of the enzyme may be the common mode of ChE inhibition by AgNPs.
Keywords
Silver nanoparticles; Acetylcholinesterase; Butyrylcholinesterase; Inhibition
Tags
IRIS
•
n-Butanol
Database searches
Pubmed
WOS
Database Searches - March 2014 (private)
Pubmed - 3/2014
WOS – 3/2014
Excluded (not pertinent)
Not chemical specific
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