US EPA

2281250 

Journal Article 

In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens 

Abbasi, MA; Rubab, K; Aziz-ur-Rehman; Riaz, T; Shahzadi, T; Khalid, M; Ajaib, M 

2012 

1 

Pakistan Journal of Pharmaceutical Sciences
ISSN: 1011-601X 

UNIV KARACHI 

KARACHI 

25 

2 

357-364 

English 

22459462 

WOS:000303301700011 

Methanolic extract of Boerhavia procumbens Bank ex Roxb. was partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially after dissolving in distilled water. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol soluble fraction. The antioxidant activity of all these fractions and the remaining aqueous fraction was evaluated by four methods such as: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity and ferric thiocyanate assay. Total phenolics were also determined. Some fractions showed noteworthy antioxidant activity. The results of the antioxidant activity revealed that the ethyl acetate soluble fraction showed the highest value of percent inhibition of DPPH (82.54 +/- 0.62) at the concentration of 125 mu g/ml. The IC50 of this fraction was 37.11 +/- 0.23 mu g/ml, compared with butylated hydroxytoluene (BHT), which have IC50 of 12.1 +/- 0.92 mu g/mL. It also showed the highest FRAP value (251.08 +/- 1.46 mu g of trolox equivalents) as well as the highest value of lipid peroxidation inhibition (57.21 +/- 52%), the highest total antioxidant activity (0.549 +/- 0.08) and also the highest total phenolic contents (77.1 +/- 0.6) as compared to the studied fractions. Phytochemical screening showed high percentage of phenolics, flavonoides and cardiac glycosides in this fraction. 

Boerhavia procumbens Bank ex Roxb.; DPPH assay; total antioxidant activity; FRAP value; inhibition of lipid peroxidation (%) and total phenolics