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HERO ID
2849947
Reference Type
Journal Article
Title
Novel antioxidant capacity assay for lipophilic compounds using electron paramagnetic resonance spectroscopy
Author(s)
Takahashi, Y; Ichimori, K; Okano, M; Goto, H
Year
2015
Is Peer Reviewed?
Yes
Journal
Journal of Clinical Biochemistry and Nutrition
ISSN:
0912-0009
EISSN:
1880-5086
Volume
56
Issue
2
Page Numbers
105-110
Language
English
PMID
25759515
DOI
10.3164/jcbn.14-36
Abstract
A novel antioxidant capacity assay for lipophilic compounds was developed using electron paramagnetic resonance (EPR) spectroscopy. The assay is based on antioxidant's scavenging ability against the tert-butoxyl radical generated photolytically from di-tert-butyl peroxide in ethyl acetate, and named the tert-butoxyl-based antioxidant capacity (BAC) assay. The radical was trapped by spin trap, 5,5-dimethyl-1-pyrroline-N-oxide, and EPR signal intensity of the spin adduct was used as a quantitative marker of radical levels. Signal intensity decreased in a dose-dependent manner in the presence of an antioxidant that competitively reacts with the radical, which was utilized to evaluate BAC values. The BAC method enabled the accurate estimation of antioxidant capacity for lipophilic materials that may counteract lipid peroxidation in biological membranes. The BAC values for quercetin and caffeic acid are 0.639 ± 0.020 and 0.118 ± 0.012 trolox equivalents, respectively, which are much smaller than values obtained by other aqueous methods such as H-ORAC and ORAC-EPR. Thus, antioxidants present in a non-aqueous environment should be evaluated using a non-aqueous system. In combination with in situ ascorbate reduction, the BAC method was capable of accurately determining the antioxidant capacity of water-insoluble materials that may be reduced in living cells.
Tags
IRIS
•
ETBE
Combined Dataset (After duplicates removed electronically)
Excluded / Not on Topic
Other chemical/non ETBE studies
OPPT
•
3-Methoxybutyl acetate
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