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Citation
Tags
HERO ID
3071037
Reference Type
Journal Article
Title
Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats
Author(s)
Abd El-Fattah, AA; Fahim, AT; Sadik, NA; Ali, BM
Year
2016
Is Peer Reviewed?
Yes
Journal
General and Comparative Endrocrinology
ISSN:
0016-6480
EISSN:
1095-6840
Publisher
Academic Press Inc.
Location
SAN DIEGO
Volume
225
Page Numbers
45-54
Language
English
PMID
26361869
DOI
10.1016/j.ygcen.2015.09.006
Web of Science Id
WOS:000366953000005
URL
http://www.sciencedirect.com/science/article/pii/S001664801500249X
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Abstract
The present study aimed to evaluate the protective role of resveratrol and curcumin on oxidative testicular damage induced by di-(2-ethylhexyl) phthalate (DEHP). Male Wistar rats were divided into six groups; three groups received oral daily doses of DEHP (2g/kgBW) for 45days to induce testicular injury. Two of these groups received either resveratrol (80mg/kgBW) or curcumin (200mg/kgBW) orally for 30days before and 45days after DEHP administration. A vehicle-treated control group was also included. Another two groups of rats received either resveratrol or curcumin alone. Oxidative damage was observed by decreased levels of total antioxidant capacity (TAC) and glutathione (GSH) and increased malondialdehyde (MDA) level in the testes of DEHP-administered rats. Serum testosterone level as well as testicular marker enzymes activities; acid and alkaline phosphatases (ACP and ALP) and lactate dehydrogenase (LDH) showed severe declines. DEHP administration caused significant increases in the testicular gene expression levels of Nrf2, HO-1, HSP60, HSP70 and HSP90 as well as a significant decrease in c-Kit protein when compared with the control group. Histopathological observations provided evidence for the biochemical and molecular analysis. These DEHP-induced pathological alterations were attenuated by pretreatment with resveratrol and curcumin. We conclude that DEHP-induced injuries in biochemical, molecular and histological structure of testis were recovered by pretreatment with resveratrol and curcumin. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties along with boosting Nrf2, HSP 60, HSP 70 and HSP 90 gene expression levels and as such may be useful potential tools in combating DEHP-induced testicular dysfunction.
Keywords
DEHP
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Not chemical specific
Use in sample prep or assay
Litsearch June 2015 - Jan 2016
Pubmed
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