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Citation
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HERO ID
3344531
Reference Type
Journal Article
Title
Cytotoxicity and genotoxicity of butyl cyclohexyl phthalate
Author(s)
Koksal, C; Nalbantsoy, A; Yavasoglu, NUK
Year
2016
Is Peer Reviewed?
Yes
Journal
Cytotechnology
ISSN:
0920-9069
EISSN:
1573-0778
Volume
68
Issue
2
Page Numbers
213-222
Language
English
PMID
25501535
DOI
10.1007/s10616-014-9770-8
Web of Science Id
WOS:000370359200004
Abstract
Butyl cyclohexyl phthalate (BCP) is frequently used in personal care products, medical and household applications. The aim of this study is therefore to evaluate possible cytotoxicity and genotoxicity of BCP using in vitro and in vivo assays. The in vitro cytotoxic effect of BCP was investigated on mouse fibroblastic cell line (L929 cells) by MTT assay. The result showed that BCP inhibits cell proliferation in a concentration-dependent manner (IC50 value = 0.29 A mu g/mL). For genotoxicity assessment, tested concentrations of BCP demonstrated mutagenic activity in the presence of S9 mix with the Salmonella strain TA100 in the Ames test. Results showed that BCP is a secondary mutagenic substance even in low concentrations. The data obtained from 28-days repeated toxicity tests on mice revealed that BCP caused abnormalities of chromosome number, in a dose-dependent manner. Additionally, DNA damage, particularly DNA strand breaks, was assessed by Comet assay. The test result shows that BCP seemed to have genotoxic potential at a high level of exposure.
Keywords
Butyl cyclohexyl phthalate; Cytotoxicity; Genotoxicity; Mutagenicity; Chromosome aberration
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Not chemical specific
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