US EPA

3859133 

Journal Article 

Role of PI3K/AKT/mTOR signaling pathway in DBP-induced apoptosis of testicular sertoli cells in vitro 

Wang, H; Wang, J; Zhang, J; Jin, S; Li, H 

2017 

Yes 

Environmental Toxicology and Pharmacology
ISSN: 1382-6689
EISSN: 1872-7077 

53 

145-150 

English 

28578144 

10.1016/j.etap.2017.05.013 

WOS:000406566200018 

http://www.sciencedirect.com/science/article/pii/S138266891730145X
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Dibutyl phthalate (DBP) has significant male reproductive toxicity, and the Sertoli cells are the target cells of DBP. This study was to investigate the injury effect induced by DBP on rat testicular Sertoli cells in vitro. MTT results showed that DBP can significantly reduce the survival rate of Sertoli cells; Hoechst staining results showed that the Sertoli cells treated with DBP emerged with typical morphological characteristics of apoptosis, nuclear condensation and chromatin condensation; flow cytometry results showed that DBP significantly increased the apoptotic rate of Sertoli cells, and dose-dependent; Western blotting showed that the expression of PTEN protein in Sertoli cells was significantly higher than that in the control group after treated with different concentrations of DBP for 24h, while the expression of p-PI3K1, p-AKT, p70S6K and 4E-BP1 protein in the PI3K/AKT/mTOR signal pathway were significantly decreased. It is speculated that PTEN/PI3K/AKT/mTOR signaling pathway plays an important role in DBP-induced apoptosis of testicular Sertoli cells in rats. 

Dibutyl phthalate; Sertoli cells; Apoptosis