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4198521 
Journal Article 
Melatonin protects oocytes from MEHP exposure-induced meiosis defects in porcine 
Yu, Z; Wang, T; Lan, M; Zang, XW; Li, YL; Cui, XS; Kim, NH; Sun, SC 
2018 
Yes 
Biology of Reproduction
ISSN: 0006-3363
EISSN: 1529-7268 
Oxford University Press 
Biol Reprod. 
98 
3 
286-298 
English 
29373650 
WOS:000428260000004 
In 2011, DEHP (plasticizer) was reported to illegally be added in food and beverage products in Taiwan, which caused great concerns about food safety worldwide. DHEP has multiple toxic effects to human and animals like endocrine disruption, cardiotoxicity, reproductive function and development defects. However, the toxic effects of DEHP on mammalian oocyte quality are still unclear. Since MEHP is the active metabolite of DEHP in vivo, in present study we used porcine oocyte as model to explore the effects of MEHP on oocyte maturation and we also studied the effects of melatonin administration on MEHP exposure-induced meiosis defects. Our results showed that exposure to MEHP significantly decreased the polar body extrusion rate in porcine oocytes. Further study showed that cell cycle progression, meiotic spindle organization and actin assembly were all disturbed after MEHP exposure. Moreover, the DNA and histone methylation levels were also affected, showing with altered 5mC and H3K4me2 levels. These results indicated that MEHP affected porcine oocyte maturation, while MEHP exposure-induced meiotic defects were all remarkably ameliorated by the administration of melatonin in porcine oocytes. We further tried to explore the causes of MEHP toxicity on oocytes, and we found that MEHP exposure resulted in significant elevations of oxidative stress and induced early apoptosis as well as elevated autophagy, while melatonin administration could reduce these. Taken together, our results indicated that MEHP exposure induced deterioration of oocyte quality, whereas melatonin supplement showed amelioration on oocyte maturation through its rescue effects on oocyte oxidative stress mediated apoptosis and autophagy. 
Cytoskeleton; Mehp; Melatonin; Oocyte; Oxidative stress; melatonin; phthalic acid 2 ethylhexyl monoester; reactive oxygen metabolite; antioxidant; melatonin; mono-(2-ethylhexyl)phthalate; phthalic acid bis(2 ethylhexyl) ester; plasticizer; reactive oxygen metabolite; animal cell; antral follicle; apoptosis; Article; autophagy; cell cycle progression; cell protection; comparative study; confocal laser scanning microscopy; confocal microscopy; controlled study; cumulus cell; DNA methylation; exposure; female; fluorescence microscopy; histone methylation; immunofluorescence; in vivo study; meiosis; meiotic spindle; mouse; nonhuman; oocyte; oocyte maturation; ovary follicle fluid; oxidative stress; polar body; priority journal; real time polymerase chain reaction; RNA isolation; Western blotting; analogs and derivatives; animal; cell cycle; cytoskeleton; drug effect; meiosis; metabolism; oocyte; oocyte development; pig; Animals; Antioxidants; Apoptosis; Autophagy; Cell Cycle; Cytoskeleton; Diethylhexyl Phthalate; Female; Meiosis; Melatonin; Oocytes; Oogenesis; Oxidative Stress; Plasticizers; Reactive Oxygen Species; Swine 
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