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HERO ID
4239555
Reference Type
Journal Article
Title
The development of mechanically formed stable nanobubbles intended for sonoporation-mediated gene transfection
Author(s)
Abdalkader, R; Kawakami, S; Unga, J; Higuchi, Y; Suzuki, R; Maruyama, K; Yamashita, F; Hashida, M
Year
2017
Is Peer Reviewed?
Yes
Journal
Drug Delivery
ISSN:
1071-7544
Volume
24
Issue
1
Page Numbers
320-327
Language
English
PMID
28165819
DOI
10.1080/10717544.2016.1250139
Web of Science Id
WOS:000395077800032
Abstract
In this study, stable nano-sized bubbles (nanobubbles [NBs]) were produced using the mechanical agitation method in the presence of perfluorocarbon gases. NBs made with perfluoropropane had a smaller size (around 400 nm) compared to that of those made with perfluorobutane or nitrogen gas. The lipid concentration in NBs affected both their initial size and post-formulation stability. NBs formed with a final lipid concentration of 0.5 mg/ml tended to be more stable, having a uniform size distribution for 24 h at room temperature and 50 h at 4 °C. In vitro gene expression revealed that NBs/pDNA in combination with ultrasound (US) irradiation had significantly higher transfection efficacy in colon C26 cells. Moreover, for in vivo gene transfection in mice left limb muscles, there was notable local transfection activity by NBs/pDNA when combined with US irradiation. In addition, the aged NBs kept at room temperature or 4 °C were still functional at enhancing gene transfection in mice. We succeeded in preparing stable NBs for efficient in vivo gene transfection, using the mechanical agitation method.
Keywords
Fluorocarbons; 9007-49-2; Index Medicus; mechanical agitation; Nano-sized bubbles; gene transfection; stability; ultrasound; Particle Size; Plasmids -- chemistry; Animals; Cell Survival -- physiology; Cell Line, Tumor; Cell Survival -- drug effects; Genetic Therapy -- methods; Mice, Inbred ICR; Plasmids -- administration & dosage; Ultrasonic Waves; Transfection -- methods; DNA -- chemistry; DNA -- administration & dosage; Mechanical Phenomena; Nanoparticles -- chemistry; Fluorocarbons -- chemistry; Nanoparticles -- administration & dosage; Fluorocarbons -- administration & dosage
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Litsearch Update 2017-2018
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