High performance liquid chromatographic determination of acetohexamide and its metabolite, hydroxyhexamide | Health & Environmental Research Online (HERO)

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Citation
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HERO ID

5096056

Reference Type

Journal Article

Title

High performance liquid chromatographic determination of acetohexamide and its metabolite, hydroxyhexamide

Author(s)

Takagishi, Y; Sato, K; Tomita, K; Sakamoto, T

Year

1979

Is Peer Reviewed?

Journal

Yakugaku Zasshi
ISSN: 0031-6903
EISSN: 1347-5231

Volume

Issue

Sep 1979

Abstract

IPA COPYRIGHT: ASHP A simple high performance liquid chromatographic assay was developed for acetohexamide (I) and its active plasma metabolite, hydroxyhexamide. The plasma sample was extracted with benzene ethyl acetate at pH 5 and the organic phase was evaporated. The 50% acetonitrile solution of the residue was chromatographed using a Lichrosorb RP-8 reverse phase column and the mobile phase was composed of 0.2% AcOH-acetonitrile (1:1). This assay method may be applicable for the bioavailability and clinical pharmacokinetic studies of I.