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5096185 
Journal Article 
Characterization of the aglycones of the toxin principle of the sea cucumber Holothuria atra 
Delia, TJ; Hertel, LW; Wittle, LW 
1977 
1 
Toxicon
ISSN: 0041-0101
EISSN: 1879-3150 
15 
5 
461-462 
HEEP COPYRIGHT: BIOL ABS. A number of biologically active compounds were isolated from the body walls and Cuvierian organs of Holothuroidea. These compounds are glycosides of tetracyclic triterpenes. H. atra is unique in that when physically disturbed it secretes a toxin burgundy colored fluid which probably serves as a defense mechanism. Separation of the aglycones was effected by preparative TLC on silica gel with benzene: ethyl acetate (9:1) as the eluent. The 2 components which were isolated were previously reported for other species and identification was aided by comparison of spectroscopic properties. 
IRIS
• Ethylbenzene
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