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Citation
Tags
HERO ID
5101419
Reference Type
Journal Article
Title
Analysis of glycerophospholipid metabolism after exposure to PCB153 in PC12 cells through targeted lipidomics by UHPLC-MS/MS
Author(s)
Wang, X; Xu, Y; Song, X; Jia, Q; Zhang, X; Qian, Y; Qiu, J
Year
2019
Is Peer Reviewed?
Yes
Journal
Ecotoxicology and Environmental Safety
ISSN:
0147-6513
EISSN:
1090-2414
Volume
169
Page Numbers
120-127
Language
English
PMID
30445242
DOI
10.1016/j.ecoenv.2018.11.006
Web of Science Id
WOS:000456226600015
Abstract
Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that have neurotoxicity, reproductive toxicity, hepatotoxicity and immunotoxicity in both animals and humans. Few studies have focused on the changes to endogenous glycerophospholipid metabolism caused by PCB153. To evaluate the relationships between exposure to PCB153 and specific endogenous glycerophospholipid metabolism, an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was implemented in this study. Twenty-two endogenous glycerophospholipids in PC12 cells were analyzed after exposure to PCB153 at dosages of 0.05 μg mL-1, 0.5 μg mL-1 or 20 μg mL-1 for 120 h. PC(14:0/14:0), PE(16:0/18:1), PE(16:0/18:2), PS(18:0/18:1) and PI(16:0/18:1) were identified as potential biomarkers under the rules of t-test (P) value < 0.05 and variable importance at projection (VIP) value > 1. It was also found that the alterations at 0.05 μg mL-1 and 20 μg mL-1 PCB153 were similar at 120 h, while 0.5 μg mL-1 PCB153 presented an opposite trend. Additionally, significant upregulation of PC, PE and PS with the same fatty acid chains of 18:0/18:2 was found after exposure to 0.05 μg mL-1 and 20 μg mL-1 PCB153 at 120 h. This study revealed that PCB153 exposure modulated 22 endogenous glycerophospholipids in PC12 cells and provided the basis for the further study of PCB153 on the effects of glycerophospholipids on PC12 cells.
Tags
IRIS
•
Methylmercury
Literature Search: Mar 2017 - May 2019
Human Data
WoS
•
PCBs
Not prioritized for screening
Litsearches
Litsearch: Aug 2018 - Aug 2019
PubMed
Not prioritized for screening
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