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Tags
HERO ID
6326326
Reference Type
Journal Article
Title
Cytotoxicity of perfluorodecanoic acid on mouse primary nephrocytes through oxidative stress: Combined analysis at cellular and molecular levels
Author(s)
Gao, S; Jing, M; Xu, M; Han, D; Niu, Q; Liu, R
Year
2020
Is Peer Reviewed?
Yes
Journal
Journal of Hazardous Materials
ISSN:
0304-3894
EISSN:
1873-3336
Publisher
Elsevier B.V.
Location
AMSTERDAM
Volume
393
Page Numbers
122444
Language
English
PMID
32169814
DOI
10.1016/j.jhazmat.2020.122444
Web of Science Id
WOS:000532829200093
Abstract
Long-chain perfluoroalkyl acids (PFAAs) such as perfluorodecanoic acid (PFDA) are toxic, persistent organic pollutants. This study investigated the harmful effect of PFDA on mouse primary nephrocytes and its mechanism at cellular and molecular levels. Cellular results showed that PFDA exhibited nephrotoxicity with decreased cell viability and increased apoptosis. The increase of intracellular reactive oxygen species (ROS) content and the decrease of intracellular superoxide dismutase (SOD) activity were significant (p < 0.01) when PFDA concentration exceeded 10 μM. Additionally, the molecular results indicated that PFDA bind with Val-A98 in the surface of Cu/Zn-SOD by a 3.11 Å hydrogen bond driven by Van der Waals' force and hydrogen bonding force, which triggered the structural changes and decreased activity of Cu/Zn-SOD. Altogether, the intracellular oxidative stress is the main driver of nephrocyte apoptosis; and the interaction of PFDA and Cu/Zn-SOD exacerbated the oxidative stress in nephrocytes, which is also a nonnegligible reason of cytotoxicity induced by PDFA. This study represented a meaningful method to explore the toxic effect and mechanism of xenobiotics at cellular and molecular levels. The findings have implications for revealing the clearance of long-chain PFAAs in vivo.
Keywords
Perfluorodecanoic acid; Nephrotoxicity; Oxidative stress; Superoxide dismutase
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch Update: November 2021
Web of Science
Perfluorooctane
•
PFAS 150
Literature Search Update December 2021
WOS
Missing 2021 searches
Literature Search Update December 2020
WOS
Literature Search August 2019
Web of Science
Perfluorinated compounds
Perfluorooctane
•
PFAS Universe
Data Source
Web of Science
Pubmed
Perfluorodecanoate
Perfluorodecanoic acid
Perfluorooctane
Perfluorooctanesulfonate
•
PFDA
Literature Search
Pubmed
WOS
Other
Published PFAS SEMs
Literature Search Update 5/2020
PubMed
Literature Search Update 4/2021
PubMed
WoS
Scopus: April 2021
June 2022 Pelch Database
Literature Searches (through April 2023 update and post-public comment/peer review)
Title & Abstract Screening
Tagged as Supplemental
In vitro and in silico models
•
PFHxS
OW - HHRAB
•
PFOA (335-67-1) and PFOS (1763-23-1)
Literature Search Update (Apr 2019 - Sep 2020)
WOS
LitSearch Update (Sept 2020 - Feb 2022)
WOS
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