US EPA

664881 

Journal Article 

Cloning, nucleotide sequence, and characterization of the genes encoding enzymes involved in the degradation of cumene to 2-hydroxy-6-oxo-7-methylocta-2,4-dienoic acid in Pseudomonas fluorescens IP01 

Aoki, H; Kimura, T; Habe, H; Yamane, H; Kodama, T; Omori, T 

1996 

1 

Journal of Fermentation and Bioengineering
ISSN: 0922-338X
EISSN: 1872-8073 

81 

3 

187-196 

English 

10.1016/0922-338X(96)82207-0 

WOS:A1996UP07100001 

http://://WOS:A1996UP07100001
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A gene cluster encoding cumene (isopropylbenzene)-degrading enzymes was cloned in Escherichia coli JM109 from newly isolated Pseudomonas fluorescens strain IP01. E. coli cells containing pIP103 (10.8-kb insert) converted cumene, other monocyclic aromatic hydrocarbons and biphenyl into the meta-cleaved compounds, whereas the cells containing pIP107D (4.8-kb insert) formed the cis-dihydrodiol compounds, and the cells containing pIP107 (5.2-kb insert) formed the catechol derivatives. We proved that this oxidation system degrades cumene using three enzymes, aromatic-ring dioxygenase, dihydrodiol dehydrogenase, and extradiol dioxygenase. Moreover we determined the nucleotide sequence of a 6,508-bp region encoding aromatic-ring dioxygenase, dihydrodiol dehydrogenase, and extradiol dioxygenase, which subsequently led to identification of six out of seven open reading frames (ORFs) from P. fluorescens IP01. T 

Nucleic acids analysis; Purines analysis; Pyrimidines analysis; Nucleic acids; Purines; Pyrimidines; Biophysics; Macromolecular systems; Molecular biology; Enzymes analysis; Bacteria physiology; Bacteria metabolism; Bacteria genetics; Viruses genetics; Biodegradation; Industrial microbiology