Health & Environmental Research Online (HERO)


Print Feedback Export to File
678967 
Journal Article 
[Study on androgenic and anti-androgenic effects of phthalate esters with the reporter gene assay using AR-EcoScreen, stable transfected CHO-K1 cells] 
Satoh, K; Nonaka, R; Ikeda, M; Satoh, T; Kamimura, H; Nagai, F 
2004 
0 
Annual Report of Tokyo Metropolitan Institute of Public Health
ISSN: 1348-9046 
55 
307-314 
Japanese 
Phthalate diesters are widely used as plasticizers to give flexibility to a variety of polyvinyl chloride-based products such as transfusion tubes, gloves and food wraps. Because they are leached from plastic over time, and are volatile lipid-sol. compds., phthalate diesters are ubiquitous environmental contaminants. When they are administered to rats, phthalate diesters rapidly metabolize into mono-phthalate esters. Some phthalate diesters were shown to have weak estrogenic activity in vitro and in vivo expts. We previously revealed the binding affinity of diethylhexyl phthalate, di-Bu phthalate and dicyclohexyl phthalate for androgen receptors in the screening tests of many chems. In this study, the androgenic and anti-androgenic effects of phthalate diesters and mono-phthalate esters were examd. by AR reporter gene assay using cell lines of stable transfection, which express AR and luciferase-based reporter genes. These results showed that none of the tested phthalate esters had androgenicity. On the other hand, androgen antagonistic activities were obsd. in di-Et phthalate, diisopropyl phthalate, di-n-hexyl phthalate, and butylbenzyl phthalate. 
IRIS
• Diethyl phthalate (DEP)
     Database searches
          Initial Litsearch
               Toxcenter
               Merged reference set
     Kept for further review
          Foreign Language