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1017429 
Journal Article 
Antiapoptotic efficacy of folic acid and vitamin B(12) against arsenic-induced toxicity 
Majumdar, S; Maiti, A; Karmakar, S; Sekhar Das, A; Mukherjee, S; Das, D; Mitra, C 
In Press 
Yes 
Environmental Toxicology
ISSN: 1520-4081
EISSN: 1522-7278 
27 
351-363 
English 
Earlier, we proposed that the ability of folic acid and vitamin B(12) to preserve systemic and mitochondrial function after short-term exposure to arsenic may prevent further progression to more permanent injury and pathological changes leading to cell death. To elucidate its mechanism, the present study examined the antiapoptotic efficacy of folic acid and vitamin B(12) against short-term arsenic exposure-induced hepatic mitochondria oxidative stress and dysfunction. Sixteen to eighteen weeks old male albino rats weighing 140-150 × g were divided into five groups: Control (A), Arsenic-treated (B), Arsenic + folic acid (C), Arsenic +vitamin B(12) (D), and Arsenic + folic acid + vitamin B(12) (E). Data generated indicated that folic acid and vitamin B(12) separately or in combination can give significant protection against alterations in oxidative stress and apoptotic marker parameters and downstream changes in mitochondria, namely pro-oxidative (NO, TBARS, OH(-)) and antioxidative defense (SOD, CAT, GSH) markers, iNOS protein expression, mitochondrial swelling, cytochrome c oxidase and Ca(2+)-ATPase activity, Ca(2+) content, caspase-3 activity. Additionally, results of hepatic cell DNA fragmentation, arsenic load of blood, hepatic tissue and urine, and histological observations, all strongly support that both these supplements have efficacy in preventing apoptotic changes and cellular damage. As the mechanisms of actions of both of these supplements are methylation related, a combined application was more effective. Results further reveal new molecular targets through which folic acid and vitamin B(12) separately or in combination work to alleviate one critical component of arsenic-induced liver injury: mitochondria dysfunction. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2010. 
arsenic; mitochondria oxidative stress; mitochondria dysfunction; mitochondria apoptotic changes; folic acid; vitamin B12 
• Arsenic Hazard ID
     1. Initial Lit Search
          PubMed
          WOS
          ToxNet
          WOS
          Considered New
     2. Lit Search Updates through Oct 2015
          WOS
          Considered
     4. Considered through Oct 2015
     6. Cluster Filter through Oct 2015
     7. Other Studies through Oct 2015
          MOA
          Susceptibility Category
               Nutritional Deficiencies (includes socioeconomic status & BMI)
          Health Effect Category
               Liver effects
• Arsenic (Inorganic)
     1. Literature
          PubMed
          Toxline, TSCATS, & DART
          Web of Science
          Lit search updates through Oct 2015
     3. Hazard ID Screening
          Other potentially supporting studies
     4. Adverse Outcome Pathways/Networks Screening
          Relevant
     5. Susceptibility Screening
          Relevant
               Animal
• Arsenic MOA
     4. Adverse Outcome Pathways
          Oxidative stress related effects (includes non-specific SH reactions)
     5. Health Effect
          Liver Effects
     1. MOA Literature Screening
          Health Effect Screening
          MOA Cluster
• Arsenic Susceptibility
     4. Susceptibility and Lifestages
          Nutritional deficiencies (includes socioeconomic status and BMI)
     5. Health Effect
          Liver Effects
     1. Susceptibility Literature Screening
          Keyword Search
     Life Stages Citation Mapping
          5%-10%