US EPA

1113369 

Journal Article 

Screening the Hemostatic Active Fraction of Artemisia annua L. In-vitro 

Wang, BC; Sui, J; Yu, ZW; Zhu, LC 

2011 

1 

Iranian Journal of Pharmaceutical Research
ISSN: 1735-0328
EISSN: 1726-6890 

10 

1 

57-62 

English 

Artemisinin extracted from Artemisia annua L. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. At present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. This paper purposed to further discover new indication of Artemisia annua L. connecting with the record of traditional medicine. We screened the hemostatic active fraction of Artemisia annua L. in-vitro by plasma recalcification time (PRT). The crude extract and the extract of n-butanol were purified by polyannde, MCI, gel column in order. Determining the part of 20% methanol fraction after column chromatography of MCI gel is the hemostatic active fraction of Artemisia annua L. The shorten rate of clotting time are followed by: crude extract of Artemisin annua L. (8.51%); the n-butanol extract (14.89%); water eluting fraction after the extract of n-butanol was purificated byipolyamide (22.11%); 20% methanol fraction after column chromatography of MCI gel (27.37%). It can provide experimental data for the clinical alication of Artemisia annua L. which can be exploited as hemostatic. This topic has a certain academic value and potential prospects on the deep research of the Artemisia annua L. resource. 

Artemisia annua L.; Crude extract; Hemostasis; In-vitro; Plasma; Recalcification time