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1342644 
Journal Article 
Protein binding interaction of warfarin and acetaminophen in presence of arsenic and of the biological system 
Alam, MA; Uddin, R; Haque, S 
2008 
Yes 
Bangladesh Journal of Pharmacology
ISSN: 1991-007X
EISSN: 1991-0088 
3 
49-54 
WOS:000267116600001 
Equilibrium dialysis was used to study in vitro binding of warfarin at physiological pH and temperature in bovine serum albumin (BSA) along with the increasing concentration of acetaminophen and there interaction with the protein in presence of arsenic. The free fractions were determined by UV spectrophotometric technique. The binding of warfarin to BSA depended on both drug and arsenic concentrations. Free warfarin concentration increased due to addition of acetaminophen which reduced the binding of warfarin to BSA. Free warfarin concentration also increased accordingly by increasing the concentra-tion of acetaminophen when only the BSA was present. When the binding site was blocked by sufficient amount of arsenic the increment of free concentration of warfarin decreased to a lower extent. This suggests that in presence of arsenic the warfarin being slowly displaced from its high affinity binding site with increasing acetamino-phen concentration. 
Acetaminophen; Arsenic; Bovine serum albumin; Warfarin