US EPA

1346433 

Journal Article 

Polyphenols of Mangifera indica modulate arsenite-induced cytotoxicity in a human proximal tubule cell line 

Garrido, G; Romiti, N; Tramonti, G; de la Fuente, F; Chieli, E 

2012 

0 

Revista Brasileira de Farmacognosia
ISSN: 0102-695X 

22 

2 

325-334 

10.1590/S0102-695X2011005000212 

WOS:000299742200012 

Inorganic arsenic is an ubiquitous environmental
contaminant able to cause severe pathologies in humans, including kidney disorders. The possible
protective effects of Mangifera indica L., Anacardiaceae, stem bark extract (MSBE) and some mango
phenols on the cytotoxicity of arsenite (As-III) in the proximal tubule cell line HK-2 was
investigated. In cells cultured for 24 h in presence of As-III, a dose-dependent loss of cell
viability occurred that was significantly alleviated by MSBE, followed by gallic acid, catechin
and mangiferin. Mangiferin complexed with Fe+++ proved more efficacious than mangiferin alone.
MSBE and pure phenols increased significantly the cell surviving fraction in clonogenic assays.
In cells pretreated with MSBE or phenols for 72 h the protection afforded by MSBE resulted
decreased in comparison with the shorter experiments. Cells pretreated with a subcytotoxic amount
of As-III or cultured in continuous presence of low concentration of mangiferin proved to be more
resistant to As-III, while cells cultured in presence of albumin resulted more sensitive. Because
all the above conditions share changes in expression/activity of P-glycoprotein (P-gp), a
transporter potentially involved in arsenic resistance, the capability of M. indica phenols in
modulating As-III-induced cytotoxicity would be at least in part dependent on their interactions
with P-gp. 

arsenic; HK-2 cells; Mangifera indica; mangiferin; P-glycoprotein; polyphenols