US EPA

1393480 

Journal Article 

Spin-Echo NMR Investigation of the Interaction of Phenyldichloroarsine with Glutathione in Intact Erythrocytes 

Dill, K; O'Connor, RJ; Mcgown, EL 

1987 

1 

Inorganica Chimica Acta
ISSN: 0020-1693
EISSN: 1873-3255 

NIOSH/00176956 

138 

2 

95-97 

The interaction of phenyldichloroarsine (696286) (PDA) with glutathione was studied in intact erythrocytes isolated from Hartley-guinea-pigs, using nuclear magnetic resonance (NMR) techniques. Erythrocytes were incubated with carbon-14 (C-14) labeled PDA. Uptake of C-14 by the cells was monitored by spin echo proton NMR spectroscopy. PDA derived C-14 activity was readily taken up by the cells with a saturation point of about 25 nanomoles PDA per 10(8) cells. Further addition of PDA increased the extracellular, but not intracellular, concentration of PDA. PDA reacted with glutathione in the erythrocytes to give an adduct containing two glutathiones for every one PDA. This was confirmed by an experiment in which graded amounts of PDA were added to intact erythrocytes, lysed erythrocytes, or solutions containing dialyzed guinea-pig hemolysate to which an appropriate amount of glutathione had been added. The resonances due to glutathione broadened until the equivalence point, where the adduct was formed, was reached. The authors conclude that PDA enters erythrocytes where it forms an adduct of PDA and glutathione in a ratio of 1:2, and they suggest that this adduct may react with hemoglobin. 

DCN-164684; Arsenic compounds; Chemical binding; Organo sulfur compounds; In vitro studies; Red blood cells; Physiological chemistry; Nuclear resonance; Laboratory animals; Phenyl compounds