Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1452501

Reference Type

Journal Article

Title

Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography

Author(s)

Tian, J; Ye, X; Shang, Y; Deng, Y; He, Kai; Li, X

Year

2012

Is Peer Reviewed?

Yes

Journal

Journal of Separation Science
ISSN: 1615-9306
EISSN: 1615-9314

Volume

Issue

Page Numbers

2659-2664

Language

English

PMID

23001886

DOI

10.1002/jssc.201200337

Web of Science Id

WOS:000309059800019

Abstract

In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing chloroform/n-butanol/methanol/water (4:1:3:2, v/v/v/v) was selected following consideration of the partition coefficient of the target compound. The crude extract (200 mg) was loaded onto a 280-mL HSCCC column and yielded 22 mg harpagoside and 31 mg angroside C with the purity of higher than 98 and 98.5%, respectively. It is feasible to isolate active compounds harpagoside and angroside C from S. ningpoensis using HSCCC.

Keywords

Angroside C; Harpagoside; High-speed counter-current hromatography; Scrophularia ningpoensis