Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859 | Health & Environmental Research Online (HERO)

Health & Environmental Research Online (HERO)

Print Feedback Export to File

This record has one attached file:

Citation
Tags

HERO ID

1936035

Reference Type

Journal Article

Title

Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859

Author(s)

Liu, W; Lin, J; Pang, X; Mi, S; Cui, S; Lin, J

Year

2013

Is Peer Reviewed?

Yes

Journal

Biotechnology and Applied Biochemistry
ISSN: 0885-4513
EISSN: 1470-8744

Volume

Issue

Page Numbers

623-628

Language

English

PMID

23980744

DOI

10.1002/bab.1110

Web of Science Id

WOS:000328684400010

Abstract

Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe(3+) ) by oxidization of ferrous iron (Fe(2+) ). The high-molecular-weight c-type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe(2+) oxidation respiratory pathway of A. ferrooxidans. To increase ferrous iron oxidation activity, a recombinant plasmid pTCYC2 containing cyc2 gene under the control of Ptac promoter was constructed and transferred into A. ferrooxidans ATCC19859. The transcriptional level of cyc2 gene was increased by 2.63-fold and Cyc2 protein expression was observed in the recombinant strain compared with the control. The ferrous iron oxidation activity and the arsenic stressed cell growth of the recombinant strain were also elevated.

Keywords

Acidithiobacillus ferrooxidans; cyc2; conjugation; ferrous iron oxidation; bioleaching