US EPA

2072785 

Journal Article 

[High-pressure liquid chromatography for the separation of lipophylic organochlorine xenobiotics from triglycerides in trace analysis] 

Rohleder, H; Staudacher, H; Sümmermann, W 

1976 

0 

Fresenius' Zeitschrift für Analytische Chemie
ISSN: 0016-1152 

279 

2 

152-153 

German 

10.1007/BF00440819 

PESTAB. Since in gas chromatographic determination of organochlorine insecticides such as DDT and related compound (PCB) the removal of interfering triglycerides is a tedious and time consuming task, a simple and quick high pressure liquid chromatographic method was devised. It is based on differential adsorption on silica gel. The chlorine containing compounds are eluted with n-hexane, followed by elution of triglycerides with acetone. The acetone is then driven off by rinsing with n-hexane and the stationary phase regenerated for the next separation. Although aluminum oxide would be more suitable for the separation of chlorinated compounds, silica gel was used because regeneration of the column is especially simple. The following materials were used: stationary phase: silica gel Merckosorb Si 60, 10 mum; mobile phase n-hexane; separation column VA-Stecl, length 40 cm, inside diameter 8 mm; column capacity 10 g; room temperature; net retention volume for DDT 5 ml; pump: Waters 6000 or LEWA type HLM 1; detector: Uvicord type 4701; recovery: 95%; time required: 45 min. The method was used over one year in PCB determinations in different types of fats, cheese, and milk.