US EPA

2151543 

Journal Article 

Genomic analysis of the phenylacetyl-CoA pathway in Burkholderia xenovorans LB400 

Patrauchan, MA; Parnell, JJ; Mcleod, MP; Florizone, C; Tiedje, JM; Eltis, LD 

2011 

Yes 

Archives of Microbiology
ISSN: 0302-8933
EISSN: 1432-072X 

193 

9 

641-650 

English 

21519854 

10.1007/s00203-011-0705-x 

WOS:000293974600003 

The phenylacetyl-CoA (Paa) catabolic pathway and genome-wide gene expression responses to phenylacetate catabolism were studied in the polychlorinated biphenyl (PCB)-degrading strain Burkholderia xenovorans LB400. Microarray and RT-qPCR analyses identified three non-contiguous chromosomal clusters of genes that are predicted to encode a complete Paa pathway that were induced up to 40-fold during growth of LB400 on phenylacetate: paaGHIJKR, paaANEBDF, and paaC. Comparison of the available genome sequences revealed that this organization is unique to Burkholderiaceae. Parallel proteomic studies identified 7 of the 14 predicted Paa proteins, most of which were detected only in phenylacetate-grown cells, but not in benzoate- or succinate-grown cells. Finally, the transcriptomic and proteomic analyses revealed the induction of at least 7 predicted catabolic pathways of aromatic compounds and some aromatic plant products (phenols, mandelate, biphenyl, C(1) compounds, mevalonate, opine, and isoquinoline), as well as an oxidative stress response and a large group of transporters. Most of these genes were not induced during growth on benzoate or biphenyl, suggesting that phenylacetate or a metabolite may act as a signal that triggers multiple physiological processes. Identifying the components of the Paa pathway is important since the pathway appears to contribute to virulence of Burkholderia pathogens. 

Phenylacetyl-CoA pathway; Burkholderia xenovorans; 2DGE proteomics; Microarray; Quantitative PCR