US EPA

2170812 

Journal Article 

Monoclonal antibody study of cytochrome P-450 isoforms in the liver of rats treated with phenobarbital, 3-methylcholanthrene, and Aroclor-1254 

Kolyada, AY 

1986 

Yes 

Bulletin of Experimental Biology and Medicine
ISSN: 0007-4888
EISSN: 1573-8221 

NIOSH/00164756 

101 

4 

445-449 

English 

10.1007/BF00834407 

WOS:A1986E561900017 

Cytochrome P450 isoform distribution in livers of Wistar-rats treated with phenobarbital (50066) (PB), 3-methylcholanthrene (56495) (MC), Aroclor-1254 (11097691) (AR), pregnenalone-carbonitrile (PC), imidazole (288324) (IM), and beta-naphthoflavone (6051872) (NF) was studied. Monoclonal antibodies developed in BALB/c-mice immunized with cytochrome P450 from rats pretreated with PB, MC, or AR were used for immunoblots of electrophoretically separated microsomes or for immunohistochemical staining of sections of treated rat liver. Initial studies with these antibodies suggested that PB induced the isoforms P450b, P450PB, and P450 alpha. MC appeared to induce the isoforms P450c and P450d. Monoclonal antibodies were tested against microsomes and liver sections from rats treated with 80mg/kg PB, 20mg/kg MC, 500mg/kg AR, 80mg/kg PC for 3 days, 200mg/kg IM for 3 days, and 80mg/kg NF for 3 days. Microsomes from rats treated with PC reacted with antibodies to P450 isoforms induced by PB, while those from NF treated rats reacted with MC induced isoforms. IM isoforms appeared to be unique in this group of compounds, while AR treated liver microsomes reacted with all monoclonal antibodies. Immunohistochemical analysis demonstrated a gradient of staining, with more intense staining in cells nearer to the central veins than the portal veins.