Degradation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

2171169

Reference Type

Journal Article

Title

Degradation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor

Author(s)

Krcmar, P; Kubatova, A; Votruba, J; Erbanova, P; Novotny, C; Sasek, V

Year

1999

Is Peer Reviewed?

Journal

World Journal of Microbiology and Biotechnology
ISSN: 0959-3993
EISSN: 1573-0972

Report Number

BIOSIS/00/28893

Volume

Issue

Page Numbers

269-276

Language

English

DOI

10.1023/A:1008994912875

Web of Science Id

WOS:000081082000015

Abstract

BIOSIS COPYRIGHT: BIOL ABS. The white rot fungus Phanerochaete chrysosporium was cultivated in a perforated plate bioreactor and the expression of activities of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) was measured. Peak activities of the two enzymes were reached close to day 11 and therefore the cultivation was terminated on that day. Extracellular proteins were concentrated and both peroxidases separated by isoelectric focusing. Degradation of technical PCB mixtures containing low and highly chlor The degradation was not substrate-specific, because no significant differences between the respective degradation rates were observed with di-, tri-, tetra-, penta-, hexa-, hepta-, and octachlorinated congeners. In contrast, MnP and LiP isolated from the above-mentioned extracellular liquid did not catalyse any degradation.

Keywords

degradation; ligninolytic enzymes; polychlorinated biphenyls; white rot fungi