US EPA

2178846 

Journal Article 

Influences of op'DDD, op'DDE and solubilization procedures on the in vitro biosynthesis of 18-hydroxycorticosterone and aldosterone by sheep adrenals 

Touitou, Y; Bogdan, A 

1978 

Yes 

International Journal of Biochemistry
ISSN: 0020-711X 

PESTAB/79/0410 

9 

9 

691-695 

English 

710697 

PESTAB. Studies were carried out to determine if o,p'-DDD (TDE) and o,p'-DDE act as steroidogenesis inhibitors in vitro when added to the culture as a powder or when dissolved first in a variety of organic solvents. The organic solvents were also tested for inhibitory effects. Labeled steroids, cofactors, reagents and various concentrations of DDD and DDE were added to sheep adrenal homogenates. Following incubation, steroids were isolated and characterized. Concentrations of 0.01 mM to 1.0 mM DDD or DDE did not decrease the symptoms of adrenal steroids; however, higher concentrations (10 mM) did have an inhibitory effect. Inhibition was the same if the compounds were added as a powder or if dissolved in a solvent. All solvents acted as inhibitors.