Comparison of uroporphyrinogen decarboxylase activities and uroporphyrin accumulation in mice, Japanese quail, and cultured chick embryo hepatocytes treated with polyhalogenated aromatic compounds | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

2195300

Reference Type

Journal Article

Title

Comparison of uroporphyrinogen decarboxylase activities and uroporphyrin accumulation in mice, Japanese quail, and cultured chick embryo hepatocytes treated with polyhalogenated aromatic compounds

Author(s)

Lambrecht, R; Sinclair, P; Bement, WJ; Sinclair, J; Carpenter, H

Year

1987

Is Peer Reviewed?

Yes

Journal

Annals of the New York Academy of Sciences
ISSN: 0077-8923
EISSN: 1749-6632

Volume

514

Issue

Page Numbers

337-338

Language

English

DOI

10.1111/j.1749-6632.1987.tb48793

Abstract

The purpose of these studies was to compare the activities of uroporphyrinogen decarboxylase (UROD) in several experimental models that have been used to investigate the mechanism by which certain polyhalogenated aromatic (PHA) compounds cause hepatic porphyria. Under these conditions, mice developed porphyria in weeks, quail in days, and chick embryo hepatocytes in hours after exposure to PHA compounds as indicated by elevated levels of uroporphyrin and heptacarboxylporphyrin. UROD activity was assayed in 10 000 x g supernatants using pentacarboxylporphyrinogen 111 as substrate (5 pM substrate, 0.3 mg protein/ml, 30 min, 37OC).