US EPA

2202075 

Book/Book Chapter 

PCB-DDT toxicity on mouse liver cells: An electron microscopic study 

Greene, WB; Walker, EM, Jr; Gadsden, RH; Klobukowski, CJ; Gale, GR; Hennigar, GR 

1973 

Intercontinental Medical Book Corp 

New York, NY 

Pesticides and the environment: A continuing controversy 

2 

137-150 

English 

is a chapter of 3221949 Pesticides and the environment: A continuing controversy

Hepatotoxicity of polychlorinated biphenyls was compared to that of DDT (50293) in mice. White ICR-mice were exposed to Aroclor-1221 (11104282), Aroclor-1242 (53469219), Aroclor-1254 (11097691), and to DDT and combinations of the two groups in total amounts of 100 parts per million (ppm) in feed for 60 days. Animals were sacrificed and liver sections were removed for light and electron microscopy. The nuclei were similar in shape and position in most groups with no nuclear changes. In many cells there was a small perinuclear zone that was devoid of endoplasmic reticulum. In mice exposed to DDT, the uniformity of the peripheral displacement of the rough endoplasmic reticulum was interrupted by incursions of dilated smooth endoplasmic reticulum. The magnitude of endoplasmic reticulum changes was most evident in Aroclor-1242 groups; the glycogen was clumped with small islands of rough endoplasmic reticulum and associated mitochondria floating in flocculent material. In groups containing DDT plus Aroclor-1242 and DDT plus Aroclor-1254, the vacuolar system changes were comparable to other reports describing hypertrophy of the smooth endoplasmic reticulum after exposure to steroids, carcinogens, and a number of drugs. In DDT and Aroclor-1221 groups, the distribution and number of microbodies were comparable to controls. Lipid deposition varied from a few small droplets in the controls to many large droplets in the experimental group; all treated groups showed some form of abnormal lipid accumulation. Mitochondria showed varying degrees of degenerative change that included swelling, reduced number, ruptured membranes, and disorganized cristae. In a majority of low power electron micrographs of experimental hepatocytes, the Golgi complex was either reduced to a few scanty membranes or was totally absent. Heavy glycogen concentrations were observed in the cytoplasm of treated groups. 

9780883720509 

8th Inter-American Conference on Toxicology and Occupational Medicine 

Miami, FL 

July 8-11, 1973