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HERO ID
2279807
Reference Type
Journal Article
Title
Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting
Author(s)
Abbasi, NA; Pervaiz, T; Hafiz, IA; Yaseen, M; Hussain, A
Year
2013
Is Peer Reviewed?
1
Journal
Journal of Zhejiang University - Science B
ISSN:
1673-1581
EISSN:
1862-1783
Volume
14
Issue
9
Page Numbers
774-784
Language
English
PMID
24009197
DOI
10.1631/jzus.B1200277
Web of Science Id
WOS:000325458100002
Abstract
In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.
Keywords
Eriobotrya japonica; Micropropagation; Sterilization of loquat; Plant growth regulator; Shoot proliferation; Rhizogenesis
Tags
IRIS
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n-Butanol
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Pubmed
WOS
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Pubmed - 3/2014
WOS – 3/2014
Excluded (not pertinent)
Not chemical specific
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Inorganic Mercury Salts (2)
Mercuric Chloride
Litsearch 1999-2018
Pubmed
WOS
Mercurous Chloride
Litsearch 1999-2018
Pubmed
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