US EPA

3071092 

Journal Article 

Determination of endocrine disrupting compounds and their metabolites in fish bile 

Ros, O; Izaguirre, JK; Olivares, M; Bizarro, C; Ortiz-Zarragoitia, M; Cajaraville, MP; Etxebarria, N; Prieto, A; Vallejo, A 

2015 

1 

Science of the Total Environment
ISSN: 0048-9697
EISSN: 1879-1026 

Elsevier B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands 

536 

261-267 

English 

26218565 

10.1016/j.scitotenv.2015.07.074 

WOS:000361189800030 

https://www.proquest.com/scholarly-journals/determination-endocrine-disrupting-compounds/docview/1746895485/se-2?accountid=171501
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This work describes a new methodology for the simultaneous determination of a large variety of emerging and persistent organic compounds and some of their metabolites in fish bile samples. The target compounds were musk fragrances, alkyl phenols, hormones, pesticides, phthalate esters and bisphenol-A, all of them with a known endocrine disrupting effect. To achieve the determination these three steps were optimized: i) an enzymatic hydrolysis of the metabolites to render the unconjugated compounds; ii) the solid phase extraction of the target analytes (Plexa cartridges 200-mg); and, iii) a clean-up of the extracts (Florisil cartridges 1-g). The samples were analyzed by gas-chromatography-mass spectrometry (GC-MS), though the polar fraction required a previous derivatization with O-bis (trimethylsilyl) trifluoroacetamide. Good apparent recoveries (63-122%), repeatability (<20%) and limits of detection (LODs) ranging between 0.04 and 459 ng/mL were obtained. This method was applied to the analysis of the target analytes in bile samples of thicklip grey mullets (Chelon labrosus) from five different populations of the Basque Coast (South East Bay of Biscay) during the period of May-June 2012. The target analytes were found at concentrations ranging from

endocrine disrupting compounds; fish bile; Chelon labrosus; solid phase extraction; gas chromatography-mass spectrometry; bioconcentration factor