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HERO ID
3859055
Reference Type
Journal Article
Title
Time-resolved immunoassay based on magnetic particles for the detection of diethyl phthalate in environmental water samples
Author(s)
Zhu, F; Mao, C; Du, D
Year
2017
Is Peer Reviewed?
Yes
Journal
Science of the Total Environment
ISSN:
0048-9697
EISSN:
1879-1026
Publisher
Elsevier B.V.
Location
AMSTERDAM
Volume
601-602
Page Numbers
723-731
Language
English
PMID
28577407
DOI
10.1016/j.scitotenv.2017.05.111
Web of Science Id
WOS:000406294900071
Abstract
Diethyl phthalate (DEP) is an extensively used phthalic acid diester (PAEs) with estrogenic activity and the potential for carcinogenic and teratogenic effects. To monitor trace DEP in environmental waters, a sensitive direct competitive time-resolved fluoroimmunoassay based on magnetic particles (MPs) as solid support was established. For the assay system, the anti-DEP antibody was oriented on the surface of the MPs using goat anti-rabbit antibody as linkers, and DEP-OVA was labeled using Eu(3+). Several physicochemical factors that potentially influence the assay performance of the proposed method were investigated in detail, including concentration of MPs, dilution of DEP-OVA-Eu(3+) and incubation time. Under the optimized conditions, the method showed: (i) low limit of detection (LOD) of 5.92ng/L; (ii) satisfactory accuracy (recoveries, 91.97-134.54%) with good reproducibility (inter-CV, 4.17-9.17%; intra-CV, 7.41-14.72%). All of which indicated that the newly established method had much higher efficiency and great potential for use in environmental water analysis for DEP. In addition, the proposed immunoassay was applied for investigation of DEP in aquatic environments at Zhenjiang City. Our results showed that DEP was detected at the concentration of 2.98-65.18ng/mL in river samples and 46.95-306.19ng/mL in wastewater treatment plants (WWTPs), which showed rather high concentrations compared with reported data. Our study provides background data important for risk assessment and contamination control of DEP in the aquatic environment of this area.
Keywords
magnetic particles; time-resolved immunoassay; diethyl phthalate
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jan 2017 - July 2017
Pubmed
LitSearch July 2017 - Sept 2018
Prior Search Overlap
Pubmed
•
Diethyl phthalate (DEP)
Database searches
Jul 2017 update
PubMed
Toxline
Jan 2020 update
PubMed
Web of Science
Excluded: No Primary Data on Health Effects
Chemical treatment/disposal/remediation
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