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Citation
Tags
HERO ID
6333656
Reference Type
Journal Article
Title
Analysis of the biodegradative and adaptive potential of the novel polychlorinated biphenyl degrader Rhodococcus sp. WAY2 revealed by its complete genome sequence
Author(s)
Garrido-Sanz, D; Sansegundo-Lobato, P; Redondo-Nieto, M; Suman, J; Cajthaml, T; Blanco-Romero, E; Martin, M; Uhlik, O; Rivilla, R
Year
2020
Is Peer Reviewed?
1
Journal
Microbial genomics
ISSN:
2057-5858
Publisher
MICROBIOLOGY SOC
Location
LONDON
Volume
0
Issue
4
Language
English
PMID
32238227
DOI
10.1099/mgen.0.000363
Web of Science Id
WOS:000531010100012
URL
https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000363
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Abstract
The complete genome sequence of Rhodococcus sp. WAY2 (WAY2) consists of a circular chromosome, three linear replicons and a small circular plasmid. The linear replicons contain typical actinobacterial invertron-type telomeres with the central CGTXCGC motif. Comparative phylogenetic analysis of the 16S rRNA gene along with phylogenomic analysis based on the genome-to-genome blast distance phylogeny (GBDP) algorithm and digital DNA-DNA hybridization (dDDH) with other Rhodococcus type strains resulted in a clear differentiation of WAY2, which is likely a new species. The genome of WAY2 contains five distinct clusters of bph, etb and nah genes, putatively involved in the degradation of several aromatic compounds. These clusters are distributed throughout the linear plasmids. The high sequence homology of the ring-hydroxylating subunits of these systems with other known enzymes has allowed us to model the range of aromatic substrates they could degrade. Further functional characterization revealed that WAY2 was able to grow with biphenyl, naphthalene and xylene as sole carbon and energy sources, and could oxidize multiple aromatic compounds, including ethylbenzene, phenanthrene, dibenzofuran and toluene. In addition, WAY2 was able to co-metabolize 23 polychlorinated biphenyl congeners, consistent with the five different ring-hydroxylating systems encoded by its genome. WAY2 could also use n-alkanes of various chain-lengths as a sole carbon source, probably due to the presence of alkB and ladA gene copies, which are only found in its chromosome. These results show that WAY2 has a potential to be used for the biodegradation of multiple organic compounds.
Keywords
Biodegradation; Complete genome; Hydrocarbons; PAH; PCB; Rhodococcus
Tags
•
Ethylbenzene
Litsearch Update April 2020
PubMed
•
Naphthalene
Database Searches
PubMed
Combined data set
Data set for title/abstract screening
Excluded - PECO criteria not met (TIAB)
January 2021 Update
PubMed
•
PCBs
Excluded
Litsearches
Litsearch: Aug 2019 - Aug 2020
PubMed
Other
•
Naphthalene (2021 Evidence mapping publication)
Database Searches
PubMed
Combined data set
Data set for title/abstract screening
Excluded – PECO criteria not met
January 2021 Update
PubMed
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