US EPA

679213 

Journal Article 

Methods for measuring mutagenicity in urine of rats dosed with [14C]di(2-ethylhexyl)phthalate 

Barber, ED; Donish, WH; Mueller, KR; Hamilton, ML; Divincenzo, GD 

1985 

1 

Toxicology
ISSN: 0300-483X
EISSN: 1879-3185 

EMICBACK/58890 

34 

3 

231-245 

English 

3975921 

10.1016/0300-483X(85)90174-X 

WOS:A1985AEL3500005 

https://search.proquest.com/docview/14187439?accountid=171501
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is related to other part(s) 1334619 Methods for measuring mutagenicity in urine of rats dosed with di(2-ethylhexyl)phthalate

Di(2-ethylhexyl)phthalate (DEHP) is extensively used as a plasticizer for vinyl plastic articles. It has been found to be positive in an NCI rodent bioassay but has generally given negative results in in vitro genotoxicity tests. We therefore decided to test the urine of rats fed [14C]DEHP for mutagenic activity in the Ames Salmonella test. The recovery of radioactivity from the urine of rats dosed with [14C]DEHP was examined by solvent extraction and XAD-2 resin absorption procedures. Both of these procedures were inadequate for quantitative recovery of urinary metabolites required for subsequent mutagenicity testing using the Ames Salmonella/microsome procedure. Recoveries of less than 5% were observed using standard solvent extraction techniques whereas the XAD-2 adsorption technique gave about 67% at high resin/urine ratios. Treatment of the urine with beta-glucuronidase/aryl sulfatase did not affect these recoveries. The direct urine plating procedure represents a viable alternative to the above concentration procedures for this phthalate ester. The effects of L-histidine and the beta-glucuronidase/aryl sulfatase preparation on the background reversion frequencies of the Ames tester strains is discussed 

Mutagenicity; Salmonella typhimurium; Salmonella/microsome assay; Urine; di-(2-ethylhexyl) phthalate; Solvent extraction; XAD-2 column chromatography; Mutagen recovery; Direct urine plating