US EPA

1183924 

Journal Article 

Differences between bligh and dyer and soxhlet extractions of PCBs and lipids from fat and lean fish muscle: Implications for data evaluation 

Ewald, G; Bremle, G; Karlsson, A 

1998 

1 

Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363 

Elsevier Ltd 

BIOSIS/98/21740 

36 

3 

222-230 

English 

10.1016/S0025-326X(97)00176-8 

WOS:000074175300014 

BIOSIS COPYRIGHT: BIOL ABS. Both Bligh and Dyer extraction using chloroform/methanol and Soxhlet extraction using hexane/acetone were employed for extracting total lipid and polychlorinated biphenyls (PCBs) from muscle tissue in four species of fish: herring (Clupea harengus), salmon (Salmo salar), cod (Gadus morhua) and Northern pike (Esox lucius), all caught in the southern part of the Baltic Sea. The Bligh and Dyer method allowed a greater amount of total lipid but a lesser amount of total PCBs to be extracted than the Soxhlet method with hexane/acetone did. For all the fish except cod, the sample wet weight PCB concentrations differed significantly for the two extraction methods. When the sample PCB was normalized to extracted total lipid, the differences in the yield of the two methods increased due to differences in total lipid yield. This was most pronounced for the lean fish (cod and pike). When the two methods were compared in terms of efficiency in the extraction of different PCBs, a re 

extraction; lipids; fish; PCB; normalization; lipophilicity