US EPA

1445454 

Journal Article 

Insulinotropic action of Citrullus colocynthis seed extracts in rat pancreatic islets 

Benariba, N; Djaziri, R; Hupkens, E; Louchami, K; Malaisse, WJ; Sener, A 

2013 

1 

Molecular Medicine Reports
ISSN: 1791-2997
EISSN: 1791-3004 

7 

1 

233-236 

English 

23128986 

10.3892/mmr.2012.1151 

WOS:000312238000042 

The present study aimed to investigate the direct in vitro effects of several distinct Citrullus colocynthis seed extracts on glucose-stimulated insulin release from pancreatic islets isolated from rats. Six extracts were tested, a crude aqueous, defatted aqueous, ethyl acetate, H2O-methanol and n-butanol extract and an extract containing a major component (fraction A) identified by gel chromatography in the ethyl acetate, n-butanol and H2O-methanol extracts. Under selected experimental conditions, the majority of extracts exhibited a positive insulinotropic action, at least when tested in the presence of 8.3 mM D-glucose. The concentration-response correlation observed with distinct extracts revealed the participation of distinct chemical compounds, including compounds with an inhibitory insulinotropic potential, in the modulation of the insulin secretory response to D-glucose. The results of the present study are relevant for further investigations which aim to identify compounds exhibiting positive insulinotropic actions. These agents may be suitable for the treatment of human diabetic subjects. 

Citrullus colocynthis extracts; glucose-stimulated insulin secretion; rat isolated pancreatic islets