US EPA

1455950 

Journal Article 

Antimalarial and free radical scavenging activities of rhizomes of Polygonatum verticillatum supported by isolated metabolites 

Khan, H; Saeed, M; Khan, M; Khan, I; Ahmad, M; Muhammad, N; Khan, A 

2012 

1 

Medicinal Chemistry Research
ISSN: 1054-2523
EISSN: 1554-8120 

SPRINGER BIRKHAUSER 

NEW YORK 

21 

7 

1278-1282 

English 

10.1007/s00044-011-9637-x 

WOS:000304463600037 

Antimalarial activity of the crude extract of Polygonatum verticillatum rhizomes and its sequentially partitioned fractions were investigated against Plasmodium falciparum. The crude extract possessed notable activity (IC50: 21.67 mu g/mL) that enhanced reasonably upon fractionation. The antiparasitic potency of the n-hexane fraction was maximum (IC50: 2.33 mu g/mL) followed by chloroform (IC50: 4.62 mu g/mL). However, the remaining fractions showed insignificant activity in the assay. The extracts of the plant showed marked scavenging activity on stable free radical, DDPH. The most potent antioxidant was the chloroform fraction (IC50: 90 mu g/mL) followed by ethyl acetate (IC50: 93 mu g/mL) and n-butanol (IC50: 95 mu g/mL) fractions. In the brine shrimps lethality test, the extracts were found nontoxic with the exception of ethyl acetate fraction (LD50: 492.846 mu g/mL). The bioactivity-guided isolation resulted into 5-hydroxymethyl-2-furaldehyde (HMF) and diosgenin which strongly supports the present experimental findings. 

Polygonatum verticillatum; Antimalarial; Antioxidant; Cytotoxicity; HMF; Diosgenin