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Citation
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HERO ID
1519072
Reference Type
Journal Article
Title
Expression of arsenic regulatory protein in Escherichia coli for selective accumulation of methylated arsenic species
Author(s)
Yang, T; Liu, J-W; Gu, C; Chen, M-L; Wang, J-H
Year
2013
Is Peer Reviewed?
1
Journal
ACS Applied Materials & Interfaces
ISSN:
1944-8244
EISSN:
1944-8252
Volume
5
Issue
7
Page Numbers
2767-2772
Language
English
PMID
23484908
DOI
10.1021/am400578y
Web of Science Id
WOS:000317549100063
Abstract
ArsR is a metalloregulatory protein with high selectivity and affinity toward arsenic. We hereby report the expression of ArsR in Escherichia coli by cell engineering, which significantly enhances the adsorption/accumulation capacity of methylated arsenic species. The ArsR-expressed E. coli cells (denoted as E. coli-ArsR ) give rise to 5.6-fold and 3.4-fold improvements on the adsorption/accumulation capacity for monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), with respect to native E. coli cells. The uptake of MMA and DMA by the E. coli-ArsR is a fast process fitting Langmuir adsorption model. It is interesting to note that the accumulation of methylated arsenic is virtually not affected by the presence of competing heavy-metal species, at least 10 times of Cd(II) and Pb(II) are tolerated for the adsorption of 1 mg L(-1) methylated arsenic. In addition, an ionic strength of up to 2 g L(-1) Na(+) poses no obvious effect on the sorption of 1 mg L(-1) MMA and DMA. Furthermore, the accumulation of MMA and DMA is less sensitive to the variation of pH value, with respect to the blank control cells. Consequently, 82.4% of MMA and 96.3% of DMA at a concentration of 50 μg L(-1) could be readily removed from aqueous medium by 12 g L(-1) of E. coli-ArsR . This illustrates a great potential for the E. coli-ArsR for selective remediation of methylated arsenic species in waters, even in the presence of a high concentration of salts.
Tags
IRIS
•
Arsenic Hazard ID
PubMed
Considered New
PubMed
Considered New
2. Lit Search Updates through Oct 2015
PubMed
Considered
7. Other Studies through Oct 2015
Other
•
Arsenic (Inorganic)
1. Literature
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
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