US EPA

1639197 

Journal Article 

Effects of di(n-butyl) and monobutyl phthalate on steroidogenesis pathways in the murine Leydig tumor cell line MLTC-1 

Chen, X; Zhou, QH; Leng, L; Chen, X; Sun, ZR; Tang, NJ 

2013 

Yes 

Environmental Toxicology and Pharmacology
ISSN: 1382-6689
EISSN: 1872-7077 

Environ Toxicol Pharmacol. 

36 

2 

332–338 

English 

23712133 

10.1016/j.etap.2013.04.013 

WOS:000324082900011 

https://search.proquest.com/docview/1733511574?accountid=171501
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Di(n-butyl) phthalate (DBP) and its active metabolite monobutyl phthalate (MBP) have been shown to disrupt reproductive organ growth. The objective of this study was to evaluate the effects of DBP/MBP on steroidogenesis in the murine Leydig tumor cell line MLTC-1 in vitro. MLTC-1 cells were incubated with various concentrations of DBP (100, 1, 0.01, and 0μmol/l in DMSO) and MBP (1000, 10, 0.1, and 0μmol/l in DMSO) for 24h. Testosterone secretion was stimulated at the lowest doses and inhibited at higher treatment doses of DBP and MBP. The mRNA levels of the side-chain cleavage enzyme (P450scc), cytochrome p450c17 (P450c17) and 3β-hydroxy-steroid dehydrogenase (3βHSD) were significantly reduced in the phthalate-exposed groups, whereas, the transcription and translation of insulin-like hormone 3 (INSL3) was affected by DBP and MBP. Alterations of the steroidogenic enzymes and INSL3 in MLTC-1 cells may be involved in the biphasic effects of DBP/MBP on androgen production. 

Di(n-butyl) phthalate; Monobutyl phthalate; Insulin-like hormone 3; Testosterone; P450 side-chain cleavage enzyme; 3 beta-Hydroxysteroid dehydrogenase