US EPA

2152409 

Journal Article 

Recombinant human AhR-mediated GUS reporter gene assays for PCB congeners in transgenic tobacco plants in comparison with recombinant mouse and guinea pig AhRs 

Shimazu, S; Kawabata, Y; Inayoshi, A; Inui, H; Ashida, H; Ohkawa, H 

2010 

Yes 

Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes
ISSN: 0360-1234
EISSN: 1532-4109 

45 

8 

741-749 

English 

20936562 

10.1080/03601234.2010.515164 

WOS:000288122100001 

Four expression plasmids for recombinant human aryl hydrocarbon receptor (hAhR) consisting of a ligand binding domain of hAhR, a DNA-binding domain of LexA and a transactivation domain of VP16 as well as β-glucuronidase (GUS) reporter genes were constructed. All the expression plasmids were transformed into tobacco plants. The selected transgenic tobacco plants were used to assay. PCB congeners showed GUS activity in a TEF-dependent manner. The selected transgenic tobacco plant XhD4V17 was compared with the transgenic tobacco plants XmD4V26 and XgD2V23 containing recombinant mouse (m) AhR-mediated GUS reporter gene expression cassette and recombinant guinea pig (g) AhR-mediated GUS reporter gene expression cassette for PCB congener-inducible GUS activity. The data revealed that the tobacco plant XgD2V23 was the most active in PCB congener-inducible GUS activity. In a 1:1 mixture of PCB126 and PCB80 a reduced PCB126-induced GUS activity was observed in plant XgD2V23, which could possibly be due to interaction between PCB126 and PCB80. 

human aryl hydrocarbon receptor (hAhR); guinea pig AhR (gAhR); polychlorinated biphenyl (PCB); reporter gene assay