US EPA

2176210 

Journal Article 

Induction of cytochromes P450 and mixed-function oxidase activity by polychlorinated-biphenyls and beta-naphthoflavone in carp (Cyprinus carpio) 

Melancon, MJ; Elcombe, CR; Vodicnik, MJ; Lech, JJ 

1981 

Yes 

Comparative Biochemistry and Physiology - Part C: Comparative Pharmacology and Toxicology
ISSN: 0742-8413 

69 

2 

219-226 

English 

6116566 

WOS:A1981MD94000008 

Hepatic microsomal ethoxyresorufin-O-deethylase and benzo(a)pyrene hydroxylase activities consistently were increased and cytochrome P450 content and ethoxycoumarin-O-deethylase activity sometimes were increased in carp injected with Aroclor 1254 or beta-naphthoflavone as compared to corn oil vehicle alone. Hepatic microsomal deethylase activities and cytochrome P450 content were not significantly elevated by injection with 2,4,2',4'-tetrachlorobiphenyl as compared to corn oil alone. Kidney microsomal deethylase activities as compared to corn oil injected were unaffected by these agents except that Aroclor 1254 significantly elevated ethoxyresorufin-O-deethylase activity. Control and induced hepatic microsomal benzo(a)pyrene hydroxylase activities were unaffected by metyrapone but were significantly inhibited by alpha-naphthoflavone added in vitro. Patterns of carp hepatic microsomal enzyme activities and responses to inhibitors resemble those seen in hepatic microsomes from 3-methylcholanthrene induced mammalian species, i.e., they resemble cytochrome P448.